Samuel Atta Kumi scored the winning goal when the two sides met in the first round of the season at the CAM Park.
The dynamics of both teams have changed since that meeting - as Karela United aim to beat the drop while Asante Kotoko continue to chase for the top spot with three matches to end the League.
Champions Asante Kotoko sit in 5th place in the League table with 47th points whiles Karela United occupy the 16th spot with 40 points.
Asante Kotoko have won two of their last five matches against Karela United -with the Aiyinase based club winning one and snatching a draw in the other.
Karela United are winless in their last two visits to the Baba Yara Sports Stadium with one defeat and a draw.
The Porcupines will rely on George Mfegue, Augustine Agyapong, Steven Mukwala, Enoch Morrison, Solomon Safo Taylor, returnee Justice Blay, Richard Boadu and Maxwell Agyemang.
Karela United coach Shaibu Tanko also expect to parade Samuel Attah Kumi, Abdul Karim Ayeh, Humin Dafie Mohammed and Augustine Randolf.
The 3pm kick off fixture will be broadcast live on StarTimes Adepa channel 247
The game will be broadcast live on Startimes Adepa channel 247