Great Ambition FC (Greater Accra), WAFA SC (Volta Region), and Kumasi Ebony FC (Ashanti Region) have won their respective Division Two Leagues and will compete in the 2025/26 Access Bank Division One League.

Former Premier League sides Berekum Arsenal and Bolga All Stars, who suffered relegation in 2013 and 2017 respectively, also earned promotion.

They join five other clubs that have qualified for the second-tier competition next season. As Regional Champions, they automatically qualify for the Access Bank Division One League, where 48 clubs compete for a Premier League slot each season.

The Access Bank Division One League is Ghana's second-tier football league, divided into three zones (Southern, Middle, and Northern sectors).

The winners of each zone earn promotion to the Ghana Premier League:

Below are the Ten Regional Champions:

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