Ghana have lost their appeal to FIFA to allow striker Asamoah Gyan play against the United States.

Gyan, 20, received his second yellow card of the World Cup after taking a penalty before the referee's whistle in the 2-0 win over the Czech Republic.

His defence - that he thought the referee had given the signal amid the crowd noise - was rejected by FIFA.

"We appealed on compassionate grounds (but) it's not supported by law," said Ghana FA spokesman Randy Abbey.

"We don't need to waste time on this. We need to focus on a replacement."

Gyan is joined on the sidelines by Sulley Muntari, who is also suspended for the game against the US.

Gyan apologised to his country for his second yellow card - the first having come in Ghana's first game against Italy.

Ghana are third in Group E on three points but just one point behind Italy going into the last round of group games.

And while Dujkovic said he had been impressed by the U.S performances to date, he was confident his side would overturn the side currently bottom of their group.

"I don't have a message for the US team - they have to worry and they will suffer," he added.

"We won't play for a draw. We don't want to rely on the results from the Czechs and Italy. We always play offensive football and we'll play to win."